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Seth Green
The Nerd / Batman / Robin / Aquaman / The Penguin / Martian Manhunter / Toyman / Scarecrow / Gorilla Grodd / Little Cheese / Swamp Thing / Abin Sur / Ganthet / Green Arrow / Hawkman / Jimmy Olsen / Various (voice)

Breckin Meyer
Superman / Mirror Master (voice)

Nathan Fillion
Green Lantern / Mr. Freeze (voice)

Alex Borstein
Wonder Woman / Giganta / Woman (voice)

Matthew Senreich
The Flash / Chillblaine / Brainiac / Wildcat (voice)

Alfred Molina
Lex Luthor / Firestorm / Mr. Banjo (voice)

Paul Reubens
The Riddler (voice)

Neil Patrick Harris
Two-Face / Black Manta (voice)

Kevin Shinick
Narrator / Captain Cold (voice)

Abraham Benrubi
Cyborg/ Kilowog / Appa Ali Apsa / Solomon Grundy (voice)

Zeb Wells
Sinestro / Swamp Thing (voice)

Megan Fox
Lois Lane (voice)

Aaron Paul
Glenn (voice)

Tara Strong
Harley Quinn / Selena Gomez (voice)

Tom Root
Icicle/B'dg (voice)