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Robbie Daymond
Spider-Man / Security Captain / Doc Ock (voice)

Ben Pronsky
Venom / Security Guard #2 / Delivery guy (voice)

Josh Keaton
Green Goblin / Police Officer / Security Guard #1 (voice)

Tara Strong
Mary Jane Watson / Scientist #2 (voice)

Jennifer Hale
Science Teacher / Scientist #1 (voice)

Laura Bailey
Gwen Stacy / Ghost-Spider / Child (voice)